Escherichia Coli (E.coli)
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Lisser and Margalit (1994) attempted to determine common structural features in Escherichia coli promoters with computer analysis. They studied four intrinsic sequence-dependent characteristics in Escherichia coli (E. coli) promoter regions: "helix stability, helix flexibility, and two conformational parameters represented by the DNA tendencies for B-Z and B-A transition" (p. 823). Quantitative measures for these properties were available for the ten dinucleotides from experimental or theoretical studies; these measures were summarized in Table 1. By summing the values of the dinucleotides along the sequence, a property value for a DNA segment was obtained (p. 824). Helix stability was evaluated by "calculating the sequence-dependent free-energy values for helix to coil transition." DNA flexibility values, and B-Z and B-A transition tendencies, were determined by experimental and theoretical studies (p. 284). The authors compiled a data set of E. coli promoter sequences to include 300 mRNA promoters whose transcriptional-start position had been defined experimentally. Redundant sequences were excluded; all promoter sequences were verified in the literature. Next, 300 non-promoter sequences were randomly selected from E. coli coding regions (p. 825). Comparison between promoter and random sequences was evaluated by a t-test (p. 825). Discriminant analysis was done with the SPSS package and a computing program developed in Fortran 7
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Some common words found in the essay are:
VMS V55, B-Z B-A, Lisser Margalit, random sequences, promoter random sequences, promoter random, transcriptional-start position, discriminant analysis, helix stability, promoter sequences, Journal Biochem, coli promoters, sequence-dependent characteristics, escherichia coli, structural characteristics, flexibility preference conformation, coli promoters computer, 825 discriminant analysis, promoters computer analysis,
Approximate Word count = 913
Approximate Pages = 4 (250 words per page)
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