Gene Transfer Technologies

RobertsÆ article gives an overview of the methods and products available for the delivery of DNA into eukaryotic cells. He describes the various products which are available and gives a brief description of how and why they work. He further discusses the advantages and disadvantages of each, and the types of situations for which each is suitable. The article does not go into a great deal of information about any of the products or techniques, and it is really designed for newcomers to the field to let them know what is available on the market and to give them some guidelines as to which products are suitable for what purposes. Roberts points out that there are so many products and methodologies available now that the field can be very confusing for those contemplating it for the first time. His article is aimed at putting some of this in order by explaining the rationale behind the methodologies.

The article points out problems which investigators may encounter, and emphasizes that none of these techniques or products is 100 percent successful, or gives 100 percent reproducible results. Roberts suggests that the investigator new to the field try several products and methods to see which one is best suited for their particular project. He also adds that they may have to combine reagents, and even add ingredients

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Approximate Word count = 965
Approximate Pages = 4 (250 words per page)

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