ocytochemical staining for Fos protein to determine if oxytocin or CRH axons are preferentially located anatomically close to neurons of the NTS which are activated by peripheral administration of CCK (30-41).
Rats were given either an anorexic dose of CCK or were injected with the injection vehicle as controls. Two hours later, they were perfused with paraformaldehyde, and immunohistochemistry performed on frozen sections of caudal brainstem using a polyclonal antibody to Fos protein and either a monoclonal antibody to oxytocin or a polyclonal antibody to CRH. The researchers were able to determine that CCK administration increased the number of Fos-positive
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